Dr. Ashwani Kumar

Dr. Raju Koorakula

Bioinformatics Scientist

Gieselbergstrasse 12/1/39, 1110, Vienna, Austria   |   raju.koorakula2018@gmail.com   |   +43-6767936361

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Expertise

NGS, Microbiome, Metagenomics, Resistome

6+ years’ experience in the fields of microbial genomics, microbiome, resistome, and bioinformatics. Particularly, I have developed extensive skills in the design, execution, and analysis of whole metagenomic and metatranscriptomic projects, including sample processing, library preparation, QC, sequencing, and data analysis.

Experience

NGS/Microbiome Scientist

University of Veterinary Medicine Vienna

[01/06/2022 – 31/01/2023], Vienna, Austria

  • Design, support and execute NGS/microbiome experiments including wet lab data.
  • Bioinformatics analysis of DNA/RNA sequence data; QC, mapping, assembly, annotation, gene expression, statistics, and visualization.
  • Study of gut resistome with focus on ARG expression and MGE role in AMR spread in livestock gut.
  • Identification and characterization of genomic variations in pathogen genomes.

PhD Scholar /Research Associate

FFoQSI GmbH

[01/02/2018 – 30/12/2022], Tulln an der Donau, Austria

  • Developed optimized pipelines for sample collection, stabilization, DNA/RNA isolation, and library prep.
  • Studied antibiotic resistance dynamics in microbial communities using metagenome & metatranscriptome sequencing.
  • Assembled novel bacterial genomes from chicken colon samples based on PacBio long-read sequencing.

Research Assistant / Master Thesis

Austrian Institute of Technology

[01/07/2015 – 31/05/2017], Tulln an der Donau, Austria

Utilization of indigenous microbial communities in diverse soils for bioremediation of PAHs. Performed DNA isolation, library prep, sequencing (Illumina MiSeq), and data analysis using QIIME2 and R.

Project member in AlveoPic project

Fachhochschule Technikum WienFachhochschule Technikum Wien

Sep 2014 - Feb 2015 · 6 mosSep 2014 to Feb 2015

  • Lung Equivalent is a mechanical-hydraulic circuit that replicates the blood circulation similar to that of blood circulation of an actual human lung. This circuit has the input blood flow pressure same as that in case of a human lung.
  • AlveoPic is the system designed to store a human lung before transplantation. Giving enough time to surgeons for surgery

Medical Sales Representative

Piramal Enterprises Limited

[01/01/2012 – 31/01/2014], Hyderabad, India

  • Generated sales through doctor and pharmacy engagement and product demonstrations.
  • Built and maintained strong business relationships and customer databases.
  • Organized trade exhibitions, conferences, and meetings.

Bachelor Thesis

MSN Labs

[04/06/2011 – 30/12/2011], Hyderabad, India

Preparation and evaluation of methocarbamol and paracetamol bi-layer tablet using analytical techniques including HPLC, AAS, dissolution and disintegration testing under IP guidelines.

Publications

    1. Metatranscriptomic analysis of the chicken gut resistome response to in-feed antibiotics and natural feed additives — Frontiers in Microbiology
    2. Storage media and RNA extraction approaches influence livestock fecal microbial RNA — PeerJ
    3. Characterization of the chicken gut microbiome using long-read sequencing — Submitted.

    Conferences / Workshops Attended

    • 10th ÖGMBT Annual Meeting, 2018, Vienna.
    • German Conference on Bioinformatics, University of Vienna, 2018.
    • FFoQSI Annual Assembly, 2018–2020, Vienna.
    • Microbiome Data Congress, 2021–2022 (Online).
    • Oral presentation at “FEED2021 – International Feed Conferences”, Vienna.
    • Presentation at FFoQSI Annual Assemblies – 2018, 2019, 2020, 2021.

Education

PhD (Dr.nat.techn), Natural Resources and Life Sciences

University of Natural Resources and Life Sciences (BOKU), Vienna, Austria (2018–2023)

Master’s in Biomedical Engineering and Sciences

University of Applied Sciences Technikum Wien (2014–2017)

Bachelor’s in Pharmacy

Osmania University, Hyderabad, India (2008–2012)

Key Achievements

  • Published research abstracts in high-impact journals.
  • Helped youngsters to build better future in Bioinformatics.

Skills

Programming Skills: Python, R, perl

Analytical Skills: Genomics, Proteomics, NGS, Microbiome, gut Resistome

Wet Lab Skills: Sample processing, library preparation, HPLC, AAS, dissolution and disintegration testing

Professional Strengths

  • Complex problem solving and critical thinking
  • Excellent time management and team coordination
  • Quick adaptability and cognitive flexibility